Sanger
sequencing is one of the most common types of DNA sequencing and often used to
sequence tiny regions of DNA - usually less than 1kb - which makes it useful
for many different applications, including PCR diagnostics and genetic
research.
Researchers and clinicians have many reasons to sequence DNA. Mapping microbial
genes to the human genome have helped researchers understand our microbial
symbiosis and identified new treatment targets for bacterial pathogens.
Pros
- Fast and easy - DNA sequencing can be very time-consuming and challenging,
especially for more significant regions of DNA. - Can isolate DNA from
different regions of the human genome - Sanger sequencing can isolate DNA from
different regions of the human genome, making it useful for genetic research.
Cons
- Less precise - Sanger sequencing is not a precise process, and the results
are not always 100% accurate. - Sanger sequencing is limited to base pairs -
For Sanger sequencing to be successful, researchers typically have to know the
DNA sequence they want to sequence. This can be not easy in specific
applications, such as clinical samples.
When to use next-generation sequencing (NGS)
Next-generation sequencing has become the most common type of DNA sequencing
due to its ability to sequence large regions of DNA and perform high-throughput
analysis.
Microbiology Tests
The Sanger sequencing technique is used to determine the sequence of an
organism's genome. It is named after Fredrick Sanger, who invented it in 1977.
The method uses DNA polymerase to amplify the region of interest, then
sequencing that product using chain termination chemistry.
Molecular biology lab services
Our core facilities for molecular biology include sequencing services and
genotyping services. We also offer microbiology tests, quantitative polymerase
chain reaction qPCR, DNA extraction, and Sanger sequencing.
Bottom line
DNA sequencing is an important technology used in many different applications.
Sanger sequencing is easy, fast, and cheap, but it can only sequence DNA free
of contaminants. Next-generation sequencing is more accurate, scalable, and can
sequence more significant regions of DNA, making it useful for high-throughput
analysis and visualization.
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